Please use this identifier to cite or link to this item:
https://hdl.handle.net/2440/52580
Citations | ||
Scopus | Web of ScienceĀ® | Altmetric |
---|---|---|
?
|
?
|
Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | May, K. | - |
dc.contributor.author | Morona, R. | - |
dc.date.issued | 2008 | - |
dc.identifier.citation | Journal of Bacteriology, 2008; 190(13):4666-4676 | - |
dc.identifier.issn | 0021-9193 | - |
dc.identifier.issn | 1098-5530 | - |
dc.identifier.uri | http://hdl.handle.net/2440/52580 | - |
dc.description.abstract | The IcsA (VirG) protein of Shigella flexneri is a polarly localized, outer membrane protein that is essential for virulence. Within host cells, IcsA activates the host actin regulatory protein, neural Wiskott-Aldrich syndrome protein (N-WASP), which in turn recruits the Arp2/3 complex, which nucleates host actin to form F-actin comet tails and initiate bacterial motility. Linker insertion mutagenesis was undertaken to randomly introduce 5-amino-acid in-frame insertions within IcsA. Forty-seven linker insertion mutants were isolated and expressed in S. flexneri Delta icsA strains. Mutants were characterized for IcsA protein production, cell surface expression and localization, intercellular spreading, F-actin comet tail formation, and N-WASP recruitment. Using this approach, we have identified a putative autochaperone region required for IcsA biogenesis, and our data suggest an additional region, not previously identified, is required for N-WASP recruitment. | - |
dc.description.statementofresponsibility | Kerrie L. May and Renato Morona | - |
dc.language.iso | en | - |
dc.publisher | Amer Soc Microbiology | - |
dc.source.uri | http://dx.doi.org/10.1128/jb.00093-08 | - |
dc.subject | Hela Cells | - |
dc.subject | Humans | - |
dc.subject | Shigella flexneri | - |
dc.subject | Actins | - |
dc.subject | Trypsin | - |
dc.subject | Bacterial Proteins | - |
dc.subject | Bacterial Outer Membrane Proteins | - |
dc.subject | DNA-Binding Proteins | - |
dc.subject | Transcription Factors | - |
dc.subject | Blotting, Western | - |
dc.subject | Mutagenesis, Insertional | - |
dc.subject | Wiskott-Aldrich Syndrome Protein | - |
dc.title | Mutagenesis of the Shigella flexneri autotransporter IcsA reveals novel functional regions involved in IcsA biogenesis and recruitment of host neural Wiscott-Aldrich syndrome protein | - |
dc.type | Journal article | - |
dc.identifier.doi | 10.1128/JB.00093-08 | - |
pubs.publication-status | Published | - |
dc.identifier.orcid | Morona, R. [0000-0001-7009-7440] | - |
Appears in Collections: | Aurora harvest 5 Microbiology and Immunology publications |
Files in This Item:
There are no files associated with this item.
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.