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https://hdl.handle.net/2440/55302
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Type: | Journal article |
Title: | Nanodissection, isolation, and PCR amplification of single DNA molecules |
Author: | Lu, J. An, H. Li, H. Li, X. Wang, Y. Li, M. Zhang, Y. Hu, J. |
Citation: | Surface and Interface Analysis, 2006; 38(6):1010-1013 |
Publisher: | John Wiley & Sons Ltd |
Issue Date: | 2006 |
ISSN: | 0142-2421 1096-9918 |
Statement of Responsibility: | Junhong Lü, Hongjie An, Haikuo Li, Xueling Li, Ying Wang, Minqian Li, Yi Zhang and Jun Hu |
Abstract: | <jats:title>Abstract</jats:title><jats:p>A unique technique for positioning, dissection, and isolation of single DNA molecules using atomic force microscopy (AFM) has been demonstrated. Full‐length genome DNA molecules were first deposited and stretched by a modified ‘Molecular Combing’ technique on a 3‐aminopropyl triethoxysilane‐coated mica substrate. A single DNA fragment was dissected from individual genome DNA strands with the AFM tip at the desired position, and then isolated (or picked up) after a special operation called ‘kneading’. All the operations including imaging, dissection, and isolation could be carried out with one tip. The isolated DNA fragment on the AFM tip could be successfully amplified by single‐molecule PCR. Some of the physical and chemical mechanisms are discussed. Copyright © 2006 John Wiley & Sons, Ltd.</jats:p> |
Keywords: | AFM single DNA molecules picking up nanomanipulation PCR amplification |
DOI: | 10.1002/sia.2337 |
Published version: | http://dx.doi.org/10.1002/sia.2337 |
Appears in Collections: | Aurora harvest 5 Earth and Environmental Sciences publications |
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