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https://hdl.handle.net/2440/55418
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Type: | Journal article |
Title: | Nano-manipulation of single DNA molecules based on atomic force microscopy |
Author: | Lu, Junhong Li, Haikuo An, Hongjie Wang, Guo-Hua Wang, Ying Li, Minqian Zhang, Yi Li, Bin Hu, Jun |
Citation: | IEEE Engineering in Medicine & Biology Society, 2005; 7:7478-7481 |
Publisher: | IEEE |
Issue Date: | 2005 |
ISSN: | 1557-170X |
School/Discipline: | School of Earth and Environmental Sciences |
Statement of Responsibility: | Lu Jun-Hong, Li Hai-Kuo, An Hong-Jie, Wang Guo-Hua, Wang Ying, Li Min -Qian, Zhang Yi, Li Bin and Hu Jun |
Abstract: | Nano-manipulation of single atoms and molecules is a critical technique in nanoscience and nanotechnology. This paper will focus on the recent development of the manipulation of single DNA molecules based on atomic force microscopy (AFM) in our laboratory. Precise manipulation has been realized including varied manipulating modes such as "cutting", "pushing", "folding", "kneading", "picking up", "dipping", etc. The cutting accuracy is dominated by the size of the AFM tip, which is usually 10 nm or less. Single DNA fragments can be cut and picked up and then amplified by single molecule PCR. Thus positioning isolation and sequencing can be performed. |
Keywords: | Nano-manipulation; Single DNA molecules; Atomicforce microscopy; Molecular combing; Molecular cutting |
DOI: | 10.1109/IEMBS.2005.1616241 |
Appears in Collections: | Earth and Environmental Sciences publications |
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