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https://hdl.handle.net/2440/57072
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Type: | Journal article |
Title: | Mechanism involved in phagocytosis and killing of Listeria monocytogenes by Acanthamoeba polyphaga |
Author: | Akya, A. Pointon, A. Thomas, C. |
Citation: | Parasitology Research, 2009; 105(5):1375-1383 |
Publisher: | Springer |
Issue Date: | 2009 |
ISSN: | 0932-0113 1432-1955 |
Statement of Responsibility: | Alisha Akya, Andrew Pointon and Connor Thomas |
Abstract: | Intra-cellular pathogen, Listeria monocytogenes, is capable of invasion and survival within mammalian cells. However, Acanthamoeba polyphaga trophozoites phagocytose and rapidly degrade Listeria cells. In order to provide more information on amoeba phagocytosis and killing mechanisms, this study used several inhibitor agents known to affect the phagocytosis and killing of bacteria by eukaryotes. Amoebae were pre-treated with mannose, cytochalasin D, wortmannin, suramin, ammonium chloride, bafilomycin A and monensin followed by co-culture with bacteria. Phagocytosis and killing of bacterial cells by amoeba trophozoites was assessed using plate counting methods and microscopy. The data presented indicates that actin polymerisation and cytoskeletal rearrangement are involved in phagocytosis of L. monocytogenes cells by A. polyphaga trophozoites. Further, both phagosomal acidification and phagosome–lysosome fusion are involved in killing and degradation of L. monocytogenes cells by A. polyphaga. However, the mannose-binding protein receptor does not play an important role in uptake of bacteria by amoeba trophozoites. In conclusion, this data reveals the similar principles of molecular mechanisms used by different types of eukaryotes in uptake and killing of bacteria. |
Keywords: | Cytoskeleton Lysosomes Phagosomes Animals Acanthamoeba Listeria monocytogenes Actins Microscopy Coculture Techniques Colony Count, Microbial Phagocytosis Hydrogen-Ion Concentration Microbial Viability Protein Multimerization |
Description: | © Springer-Verlag 2009 |
DOI: | 10.1007/s00436-009-1565-z |
Published version: | http://dx.doi.org/10.1007/s00436-009-1565-z |
Appears in Collections: | Aurora harvest Molecular and Biomedical Science publications |
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