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https://hdl.handle.net/2440/6007
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dc.contributor.author | Parnas, I. | - |
dc.contributor.author | Rashkovan, G. | - |
dc.contributor.author | Ong, J. | - |
dc.contributor.author | Kerr, D. | - |
dc.date.issued | 1999 | - |
dc.identifier.citation | Journal of Neurophysiology, 1999; 81(3):1184-1191 | - |
dc.identifier.issn | 0022-3077 | - |
dc.identifier.issn | 1522-1598 | - |
dc.identifier.uri | http://hdl.handle.net/2440/6007 | - |
dc.description.abstract | Release of excitatory transmitter from boutons on crayfish nerve terminals was inhibited by (R,S)-baclofen, an agonist at GABAB receptors. Baclofen had no postsynaptic actions as it reduced quantal content without affecting quantal amplitude. The effect of baclofen increased with concentration producing 18% inhibition at 10 microM; EC50, 50% inhibition at 30 microM; maximal inhibition, 85% at 100 microM and higher. There was no desensitization, even with 200 or 320 microM baclofen. Phaclofen, an antagonist at GABAB receptors, competitively antagonized the inhibitory action of baclofen (KD = 50 microM, equivalent to a pA2 = 4.3 +/- 0.1). Phaclofen on its own at concentrations below 200 microM had no effect on release, whereas at 200 microM phaclofen itself increased the control level of release by 60%, as did 2-hydroxy-saclofen (200 microM), another antagonist at GABAB receptors. This increase was evidently due to antagonism of a persistent level of GABA in the synaptic cleft, since the effect was abolished by destruction of the presynaptic inhibitory fiber, using intra-axonal pronase. We conclude that presynaptic GABAB receptors, with a pharmacological profile similar to that of mammalian GABAB receptors, are involved in the control of transmitter release at the crayfish neuromuscular junction. | - |
dc.description.statementofresponsibility | Itzchak Parnas, Grigory Rashkovan, Jennifer Ong and David I. B. Kerr | - |
dc.language.iso | en | - |
dc.publisher | American Physiological Society | - |
dc.rights | © 1999 the American Physiological Society | - |
dc.source.uri | http://jn.physiology.org.proxy.library.adelaide.edu.au/content/81/3/1184.full | - |
dc.subject | Axons | - |
dc.subject | Neuromuscular Junction | - |
dc.subject | Animals | - |
dc.subject | Astacoidea | - |
dc.subject | Baclofen | - |
dc.subject | Pronase | - |
dc.subject | Receptors, GABA-B | - |
dc.subject | Receptors, Presynaptic | - |
dc.subject | GABA Agonists | - |
dc.subject | GABA Antagonists | - |
dc.subject | Patch-Clamp Techniques | - |
dc.subject | Logistic Models | - |
dc.subject | Neural Inhibition | - |
dc.subject | Quantum Theory | - |
dc.title | Tonic activation of presynaptic GABA (B) receptors in the opener neuromuscular junction of crayfish | - |
dc.type | Journal article | - |
dc.identifier.doi | 10.1152/jn.1999.81.3.1184 | - |
pubs.publication-status | Published | - |
dc.identifier.orcid | Ong, J. [0000-0002-0958-460X] | - |
Appears in Collections: | Anaesthesia and Intensive Care publications Aurora harvest 5 |
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