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https://hdl.handle.net/2440/60842
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Type: | Journal article |
Title: | Optimization of the non-invasive 13C-sucrose breath test in a rat model of methotrexate-induced mucositis |
Author: | Tooley, K. Howarth, G. Lymn, K. Butler, R. |
Citation: | Cancer Chemotherapy and Pharmacology, 2010; 65(5):913-921 |
Publisher: | Springer |
Issue Date: | 2010 |
ISSN: | 0344-5704 1432-0843 |
Statement of Responsibility: | K. L. Tooley, G. S. Howarth, K. A. Lymn and R. N. Butler |
Abstract: | PURPOSE: In order to determine the sensitivity and specificity of the test and to optimize experimental conditions utilizing the SBT in a rat model of chemotherapy-induced small intestinal damage. METHODS: Initially, a 13C-sucrose dose-response study was performed in rats to determine an optimal sucrose concentration for the SBT; then applied to assess chemotherapy-induced intestinal damage. A further study was conducted to establish a SBT time-course of methotrexate-induced small intestinal damage and repair. Animals were killed at 96 or 144 h. RESULTS: A sucrose concentration of 0.25 g/ml was optimal (20% CV) for reproducibility and detection of intestinal damage. Maximal damage occurred at 72 h, small intestinal repair was initiated by 96 h and continued at 144 h post-MTX, as determined by the SBT and confirmed by biochemical analyses. Levels of sensitivity and specificity for the SBT were 98 and 94%, respectively. CONCLUSIONS: The SBT is a reliable non-invasive marker of small intestinal health and damage with a high degree of sensitivity and specificity. |
Keywords: | Sucrose breath test reproducibility specificity mucositis time-course chemotherapy methotrexate |
Rights: | © Springer-Verlag 2009 |
DOI: | 10.1007/s00280-009-1098-2 |
Published version: | http://dx.doi.org/10.1007/s00280-009-1098-2 |
Appears in Collections: | Aurora harvest Molecular and Biomedical Science publications |
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