Please use this identifier to cite or link to this item: https://hdl.handle.net/2440/62178
Citations
Scopus Web of Science® Altmetric
?
?
Full metadata record
DC FieldValueLanguage
dc.contributor.authorCastanheira, M.-
dc.contributor.authorWatters, A.-
dc.contributor.authorBell, J.-
dc.contributor.authorTurnidge, J.-
dc.contributor.authorJones, R.-
dc.date.issued2010-
dc.identifier.citationAntimicrobial Agents and Chemotherapy, 2010; 54(9):3614-3617-
dc.identifier.issn0066-4804-
dc.identifier.issn1098-6596-
dc.identifier.urihttp://hdl.handle.net/2440/62178-
dc.description.abstractAmong 4,167 Staphylococcus aureus and 790 coagulase-negative Staphylococcus (CoNS; not S. saprophyticus) isolates collected consecutively from North American and Australian hospitals, only 87 (1.7% overall) isolates displayed a fusidic acid (FA; also known as CEM-102) MIC of > or = 2 microg/ml (FA resistance). These strains were further evaluated with a multiplex PCR to amplify the acquired resistance genes fusB, fusC, and fusD. Mutations in fusA and fusE were evaluated in all isolates showing an absence of acquired resistance genes and/or showing FA MIC values of > or = 64 microg/ml. S. aureus resistance rates were very low in the United States (0.3%) and were higher in Canada and Australia (7.0% for both countries). Among CoNS isolates, FA resistance rates were significantly more elevated than that for S. aureus (7.2 to 20.0%; the highest rates were in Canada). All 52 (41 CoNS) FA-resistant isolates from the United States showed FA MIC results of < or = 64 microg/ml, and 7 of 11 S. aureus isolates carried fusC. CoNS strains from the United States carried fusB or fusC. In Canada, fusB and fusC occurrences were similar among S. aureus and CoNS isolates, and modestly elevated FA MIC values were observed (all MIC results were < or = 32 microg/ml). Isolates from Australia showed MIC values ranging from 2 to 32 microg/ml, and S. aureus isolates were predominantly fusC positive. fusA mutations were detected in only three S. aureus isolates, conferring FA MIC values of 2 to 8 microg/ml. Target mutations have been considered the primary FA resistance mechanism among Staphylococcus spp.; however, acquired resistance genes appear to have a dominant role in resistance against this older antimicrobial agent. In summary, this study shows that acquired genes are highly prevalent among FA-resistant strains (>90%) in three nations with distinct or absence (United States) of fusidic acid clinical use.-
dc.description.statementofresponsibilityMariana Castanheira, Amy A. Watters, Jan M. Bell, John D. Turnidge, and Ronald N. Jones-
dc.language.isoen-
dc.publisherAmer Soc Microbiology-
dc.rightsCopyright © 2010, American Society for Microbiology. All Rights Reserved.-
dc.source.urihttp://dx.doi.org/10.1128/aac.01390-09-
dc.subjectStaphylococcus-
dc.subjectFusidic Acid-
dc.subjectBacterial Proteins-
dc.subjectAnti-Bacterial Agents-
dc.subjectMicrobial Sensitivity Tests-
dc.subjectDrug Resistance, Bacterial-
dc.subjectMutation-
dc.subjectNorth America-
dc.subjectAustralia-
dc.titleFusidic acid resistance rates and prevalence of resistance mechanisms among staphyloccoccus spp. isolated in North America and Australia (2007-2008)-
dc.typeJournal article-
dc.identifier.doi10.1128/AAC.01390-09-
pubs.publication-statusPublished-
dc.identifier.orcidTurnidge, J. [0000-0003-4240-5578]-
Appears in Collections:Aurora harvest 5
Molecular and Biomedical Science publications

Files in This Item:
There are no files associated with this item.


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.