Please use this identifier to cite or link to this item:
https://hdl.handle.net/2440/64433
Citations | ||
Scopus | Web of Science® | Altmetric |
---|---|---|
?
|
?
|
Type: | Journal article |
Title: | A chimeric GB virus B encoding the hepatitis C virus hypervariable region 1 is infectious in vivo |
Author: | Haqshenas, G. Dong, X. Netter, H. Torresi, J. Gowans, E. |
Citation: | Journal of General Virology, 2007; 88(3):895-902 |
Publisher: | Soc General Microbiology |
Issue Date: | 2007 |
ISSN: | 0022-1317 1465-2099 |
Statement of Responsibility: | G. Haqshenas, X. Dong, H. Netter, J. Torresi and E. J. Gowans |
Abstract: | Two GB virus B (GBV-B) chimeric genomes, GBV-HVR and GBV-HVRh (with a hinge), containing the coding region of the immunodominant hypervariable region 1 (HVR1) of the E2 envelope protein of Hepatitis C virus (HCV) were constructed. Immunoblot analysis confirmed that HVR1 was anchored to the GBV-B E2 protein. To investigate the replication competence and in vivo stability of in vitro-generated chimeric RNA transcripts, two naïve marmosets were inoculated intrahepatically with the transcripts. The GBV-HVR chimeric genome was detectable for 2 weeks post-inoculation (p.i.), whereas GBV-HVRh reverted to wild type 1 week p.i. Sequencing analysis of the HVR1 and flanking regions from GBV-HVR RNA isolated from marmoset serum demonstrated that the HVR1 insert remained unaltered in the GBV-HVR chimera for 2 weeks. Inoculation of a naïve marmoset with serum collected at 1 week p.i. also resulted in viraemia and confirmed that the serum contained infectious particles. All animals cleared the infection by 3 weeks p.i. and remained negative for the remaining weeks. The chimera may prove useful for the in vivo examination of any HCV HVR1-based vaccine candidates. |
Keywords: | Serum Animals Callithrix GB virus B Hepacivirus Viremia Flaviviridae Infections Viral Proteins RNA, Viral Immunoblotting Sequence Analysis, DNA Recombination, Genetic Amino Acid Sequence Base Sequence Genome, Viral Female |
Rights: | © 2007 SGM |
DOI: | 10.1099/vir.0.82467-0 |
Published version: | http://dx.doi.org/10.1099/vir.0.82467-0 |
Appears in Collections: | Aurora harvest Surgery publications |
Files in This Item:
There are no files associated with this item.
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.