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https://hdl.handle.net/2440/66939
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Type: | Journal article |
Title: | Application and adaptation of the in vitro micronucleus assay for the assessment of nutritional requirements of cells for DNA damage prevention |
Author: | Bull, C. Beetstra, S. Benassi-Evans, B. Crott, J. Kimura, M. Teo, T. Wu, J. Fenech, M. |
Citation: | Mutagenesis, 2011; 26(1):193-197 |
Publisher: | Oxford Univ Press |
Issue Date: | 2011 |
ISSN: | 0267-8357 1464-3804 |
Statement of Responsibility: | Caroline F. Bull, Sasja Beetstra-Hill, Bianca J. Benassi-Evans, Jimmy W. Crott, Michiyo Kimura, Theodora Teo, Jing Wu and Michael F. Fenech |
Abstract: | DNA damage is a fundamental cause of developmental and degenerative diseases. The in vitro cytokinesis-block micronucleus cytome (CBMN-Cyt) assay is an established comprehensive method for assessing cytostasis and chromosome stability in cells. Originally developed to study the acute effects of single environmental genotoxicants, creative applications and adaptations to the basic protocol have allowed its use in evaluating the impacts of dietary micronutrients and micronutrient combinations (nutriomes) on DNA damage. In this review, we examine some of these studies and the important findings they have generated with respect to nutrient/nutrient, nutrient/genotype and nutrient/genotoxicant interactions, as well as assessment of the carcinogenic (or protective) potential of whole dietary patterns. In addition, we outline current knowledge gaps and technical limitations and propose future adaptations to enhance the applicability of the CBMN-Cyt method for in vivo predictions. |
Keywords: | Cells, Cultured Micronuclei, Chromosome-Defective Animals Humans Rats DNA Damage Micronutrients Diet Micronucleus Tests Nutritional Requirements Gamma Rays Food |
Rights: | © The Author 2010. |
DOI: | 10.1093/mutage/geq065 |
Published version: | http://dx.doi.org/10.1093/mutage/geq065 |
Appears in Collections: | Aurora harvest 5 Molecular and Biomedical Science publications |
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