Please use this identifier to cite or link to this item:
https://hdl.handle.net/2440/70933
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Type: | Journal article |
Title: | Disruption of Trichoderma reesei cre2, encoding an ubiquitin C-terminal hydrolase, results in increased cellulase activity |
Author: | Denton, J. Kelly, J. |
Citation: | BMC Biotechnology, 2011; 11(1):103-1-103-10 |
Publisher: | BioMed Central Ltd. |
Issue Date: | 2011 |
ISSN: | 1472-6750 1472-6750 |
Statement of Responsibility: | Jai A Denton and Joan M Kelly |
Abstract: | Background: The filamentous fungus Trichoderma reesei (Hypocrea jecorina) is an important source of cellulases for use in the textile and alternative fuel industries. To fully understand the regulation of cellulase production in T. reesei, the role of a gene known to be involved in carbon regulation in Aspergillus nidulans, but unstudied in T. reesei, was investigated. Results: The T. reesei orthologue of the A. nidulans creB gene, designated cre2, was identified and shown to be functional through heterologous complementation of a creB mutation in A. nidulans. A T. reesei strain was constructed using gene disruption techniques that contained a disrupted cre2 gene. This strain, JKTR2-6, exhibited phenotypes similar to the A. nidulans creB mutant strain both in carbon catabolite repressing, and in carbon catabolite derepressing conditions. Importantly, the disruption also led to elevated cellulase levels. Conclusions: These results demonstrate that cre2 is involved in cellulase expression. Since the disruption of cre2 increases the amount of cellulase activity, without severe morphological affects, targeting creB orthologues for disruption in other industrially useful filamentous fungi, such as Aspergillus oryzae, Trichoderma harzianum or Aspergillus niger may also lead to elevated hydrolytic enzyme activity in these species. |
Keywords: | Aspergillus niger Aspergillus oryzae Trichoderma Cellulose Ubiquitin Thiolesterase Cellulase Genetic Complementation Test Transduction, Genetic Mutagenesis, Insertional Industrial Microbiology Gene Expression Regulation, Fungal Up-Regulation Amino Acid Sequence Sequence Homology, Amino Acid Plasmids Molecular Sequence Data |
Description: | The electronic version of this article is the complete one and can be found online at: http://www.biomedcentral.com/1472-6750/11/103 |
Rights: | © 2011 Denton and Kelly; licensee BioMed Central Ltd.This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
DOI: | 10.1186/1472-6750-11-103 |
Published version: | http://www.biomedcentral.com/1472-6750/11/103 |
Appears in Collections: | Aurora harvest 5 Molecular and Biomedical Science publications |
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hdl_70933.pdf | Published version | 621.4 kB | Adobe PDF | View/Open |
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