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https://hdl.handle.net/2440/7288
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Type: | Journal article |
Title: | Cloning of human lymphocyte-specific interferon regulatory factor (hLSIRF/hIRF4) and mapping of the gene to 6p23-p25 |
Author: | Grossman, A. Mittrucker, H.W. Nicholl, J. Suzuki, A. Chung, S. Antonio, L. Suggs, S. Sutherland, G. Siderovski, D. Mak, T. |
Citation: | Genomics, 1996; 37(2):229-233 |
Publisher: | ACADEMIC PRESS INC JNL-COMP SUBSCRIPTIONS |
Issue Date: | 1996 |
ISSN: | 0888-7543 1089-8646 |
Statement of Responsibility: | Grossman, Alex ; Mittrücker, Hans-Willi ; Nicholl, Jillian ; Suzuki, Akira ; Chung, Stephen ; Antonio, Laarni ; Suggs, Sid ; Sutherland, Grant R. ; Siderovski, David P. ; Mak, Tak W. |
Abstract: | The interferon regulatory factor (IRF) genes encode a family of transcription factors involved in the transcriptional regulation of interferon and the interferon stimulated genes through recognition of the interferon stimulated response element. We previously reported the cloning of a murine lymphocyte-specific IRF (mLSIRF), which was rapidly induced following B- or T-cell receptor crosslinking. To study the role of LSIRF in human lymphocyte development, we have cloned the complete 5.3-kb cDNA for the human homolog (hLSIRF). hLSIRF is a protein of 450 amino acids with a predicted molecular weight of 51.6 kDa and possesses 92% identity at the amino acid level to mLSIRF, including near identity in the DNA-binding domain. In Northern blot analysis, a single transcript of approximately 5 kb was highly expressed in spleen and peripheral blood lymphocyte. hLSIRF mRNA was rapidly induced in peripheral T cells after crosslinking the T-cell receptor. Analysis of tumor cell lines showed that hLSIRF mRNA was basally expressed in most B- but not T-cell lines. Surprisingly hLSIRF mRNA was also found in the melanoma line G361 and is expressed in normal melanocytes as well. Sequence from a genomic clone for hLSIRF was compared to that from mouse and revealed an identical exon-intron structure and a conserved PU-1-binding motif in the promoter. By FISH analysis, hLSIRF was mapped to 6p23-p25. |
Keywords: | Hela Cells Tumor Cells, Cultured Chromosomes, Human, Pair 6 Animals Humans Mice DNA-Binding Proteins Transcription Factors DNA, Complementary In Situ Hybridization, Fluorescence Chromosome Mapping Cloning, Molecular Binding Sites Amino Acid Sequence Base Sequence Molecular Sequence Data Interferon Regulatory Factors Promoter Regions, Genetic |
DOI: | 10.1006/geno.1996.0547 |
Published version: | http://dx.doi.org/10.1006/geno.1996.0547 |
Appears in Collections: | Aurora harvest 5 Paediatrics publications |
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