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https://hdl.handle.net/2440/73132
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Type: | Journal article |
Title: | Successful in vitro culture of pre-antral follicles derived from vitrified murine ovarian tissue: oocyte maturation, fertilization, and live births |
Author: | Wang, X. Catt, S. Pangesto, M. Temple-Smith, P. |
Citation: | Reproduction, 2011; 141(2):183-191 |
Publisher: | Bio Scientifica Ltd |
Issue Date: | 2011 |
ISSN: | 1470-1626 1741-7899 |
Statement of Responsibility: | Xiaoqian Wang, Sally Catt, Mulyoto Pangestu and Peter Temple-Smith |
Abstract: | Cryopreservation of ovarian tissue is an important option for preserving the fertility of cancer patients undergoing chemotherapy and radiotherapy. In this study, we examined the viability and function of oocytes derived in vitro from pre-antral follicles as an alternative method for restoring fertility. Pre-antral follicles (specified as secondary follicle with a diameter around 100–130 μm) were mechanically isolated from vitrified-warmed and fresh adult mouse ovarian tissues and cultured for 12 days followed by an ovulation induction protocol at the end of this period to initiate oocyte maturation. Oocytes were then released from these follicles, fertilized in vitro, and cultured to the blastocyst stage and vitrified. After storage in liquid nitrogen for 2 weeks, groups of vitrified blastocysts were warmed and transferred into pseudo-pregnant recipient females. Although most of the isolated mouse pre-antral follicles from fresh (79.4%) and vitrified (75.0%) ovarian tissues survived the 12-day in vitro culture period, significantly fewer mature oocytes developed from vitrified-warmed pre-antral follicles than from the fresh controls (62.2 vs 86.4%, P<0.05). No difference was observed in embryo cleavage rates between these two groups, but the proportion of embryos that developed into blastocysts in the vitrification group was only half that of the controls (24.2 vs 47.2%, P<0.05). Nevertheless, live births of healthy normal pups were achieved after transfer of vitrified blastocysts derived from both experimental groups. This study shows that successful production of healthy offspring using an in vitro follicle culture system is feasible, and suggests that this procedure could be used in cancer patients who wish to preserve their fertility using ovarian tissue cryopreservation. |
Keywords: | Ovarian Follicle Oocytes Cells, Cultured Animals Mice, Inbred C57BL Mice, Inbred CBA Animals, Newborn Mice Cryopreservation Embryo Transfer Fertilization in Vitro Cell Differentiation Oogenesis Fertilization Pregnancy Female Male Live Birth Embryo, Mammalian Vitrification |
Rights: | © 2011 Society for Reproduction and Fertility |
DOI: | 10.1530/REP-10-0383 |
Published version: | http://dx.doi.org/10.1530/rep-10-0383 |
Appears in Collections: | Aurora harvest Obstetrics and Gynaecology publications |
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