Please use this identifier to cite or link to this item:
https://hdl.handle.net/2440/74144
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DC Field | Value | Language |
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dc.contributor.author | Edelstein, P. | - |
dc.contributor.author | Edelstein, M. | - |
dc.contributor.author | Shephard, L. | - |
dc.contributor.author | Ward, K. | - |
dc.contributor.author | Ratcliff, R. | - |
dc.date.issued | 2012 | - |
dc.identifier.citation | International Journal of Systematic and Evolutionary Microbiology, 2012; 62(8):1766-1771 | - |
dc.identifier.issn | 1466-5026 | - |
dc.identifier.issn | 1466-5034 | - |
dc.identifier.uri | http://hdl.handle.net/2440/74144 | - |
dc.description | The GenBank/EMBL/DDBJ accession numbers for the 16S rRNA, mip, proA, rnpB and rpoB gene sequences of strain IMVS-3376T are HQ398202–HQ398206, respectively. | - |
dc.description.abstract | Legionella-like bacteria were isolated from the respiratory tract of two patients in California, USA, and South Australia, but were not thought to cause disease. These bacteria, strains F2632 and IMVS-3376T, were found to have identical Legionella macrophage infectivity potentiator (mip) gene sequences and were therefore further characterized to determine their genetic and phenotypic relatedness and properties. Both of these Gram-negative-staining bacterial strains grew on buffered charcoal yeast extract medium, were cysteine auxotrophs and made a characteristic diffusible bright yellow fluorescent pigment, with one strain making a late appearing colony-bound blue–white fluorescent pigment. The optimal in vitro growth temperature was 35 °C, with very poor growth at 37 °C in broth or on solid media. There was no growth in human A549 cells at either 35 or 37 °C, but excellent growth in Acanthamoeba castellani at 30 °C and poorer growth at 35 °C. Phylogenetic analysis of these bacteria was performed by sequence analysis of 16S rRNA, mip, ribonuclease P, ribosomal polymerase B and zinc metalloprotease genes. These studies confirmed that the new strains represented a single novel species of the genus Legionella for which the name Legionella steelei sp. nov. is proposed. The type strain is IMVS-3376T ( = IMVS 3113T = ATCC BAA-2169T). | - |
dc.description.statementofresponsibility | Paul H. Edelstein, Martha A. Edelstein, Lisa J. Shephard, Kevin W. Ward and Rodney M. Ratcliff. | - |
dc.language.iso | en | - |
dc.publisher | Soc General Microbiology | - |
dc.rights | Copyright © 2012 International Union of Microbiological Societies | - |
dc.source.uri | http://dx.doi.org/10.1099/ijs.0.035709-0 | - |
dc.subject | Respiratory System | - |
dc.subject | Cell Line | - |
dc.subject | Humans | - |
dc.subject | Legionella | - |
dc.subject | DNA, Bacterial | - |
dc.subject | RNA, Ribosomal, 16S | - |
dc.subject | Pigmentation | - |
dc.subject | Sequence Analysis, DNA | - |
dc.subject | Temperature | - |
dc.subject | Phylogeny | - |
dc.subject | Genotype | - |
dc.subject | Molecular Sequence Data | - |
dc.subject | Adult | - |
dc.subject | California | - |
dc.subject | South Australia | - |
dc.subject | Male | - |
dc.title | Legionella steelei sp. nov., isolated from human respiratory specimens in California, USA, and South Australia | - |
dc.type | Journal article | - |
dc.identifier.doi | 10.1099/ijs.0.035709-0 | - |
pubs.publication-status | Published | - |
Appears in Collections: | Aurora harvest Microbiology and Immunology publications |
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