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https://hdl.handle.net/2440/74946
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Type: | Journal article |
Title: | A new methodology for differentiation and typing of closely related Salmonella enterica serovar Heidelberg isolates |
Author: | Young, C. Ross, I. Heuzenroeder, M. |
Citation: | Current Microbiology: an international journal, 2012; 65(5):481-487 |
Publisher: | Springer |
Issue Date: | 2012 |
ISSN: | 0343-8651 1432-0991 |
Statement of Responsibility: | Chun-Chun Young, Ian L. Ross, Michael W. Heuzenroeder |
Abstract: | his study describe the use of a combination of two recently proposed typing approaches, multiple amplification of prophage locus typing (MAPLT) and multiple-locus variable-number tandem-repeat analysis (MLVA) for subdividing within Salmonella enterica serovar Heidelberg (S. Heidelberg). The combined typing method was compared with pulsed-field gel electrophoresis (PFGE) by Simpson’s index of diversity (DI). PFGE was shown to have a DI = 0.84 and was poor at differentiation of the predominant PT1 (Phage Type 1) phenotype. In comparison, the combined MAPLT/MLVA method comprising 3 MLVA and 9 MAPLT primer pairs provided a higher differentiating ability DI = 0.92. More importantly, the combined methodology was found to be superior in the differentiation of the predominant PT1 isolates. In conclusion, this study demonstrated the potential of the rapid and simple amalgamated MAPLT/MLVA approach in determining transmission of isolates of clonal phage type groups from various environmental sources to humans. |
Keywords: | Molecular typing MLVA MAPLT Salmonella Heidelberg |
Rights: | © Springer Science+Business Media, LLC 2012 |
DOI: | 10.1007/s00284-012-0179-3 |
Grant ID: | NHMRC |
Published version: | http://dx.doi.org/10.1007/s00284-012-0179-3 |
Appears in Collections: | Aurora harvest Molecular and Biomedical Science publications |
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