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https://hdl.handle.net/2440/75447
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Type: | Journal article |
Title: | Streptococcus pneumoniae uses glutathione to defend against oxidative stress and metal ion toxicity |
Author: | Potter, A. Trappetti, C. Paton, J. |
Citation: | Journal of Bacteriology, 2012; 194(22):6248-6254 |
Publisher: | Amer Soc Microbiology |
Issue Date: | 2012 |
ISSN: | 0021-9193 1098-5530 |
Statement of Responsibility: | Adam J. Potter, Claudia Trappetti, and James C. Paton |
Abstract: | The thiol-containing tripeptide glutathione is an important cellular constituent of many eukaryotic and prokaryotic cells. In addition to its disulfide reductase activity, glutathione is known to protect cells from many forms of physiological stress. This report represents the first investigation into the role of glutathione in the Gram-positive pathogen Streptococcus pneumoniae. We demonstrate that pneumococci import extracellular glutathione using the ABC transporter substrate binding protein GshT. Mutation of gshT and the gene encoding glutathione reductase (gor) increases pneumococcal sensitivity to the superoxide generating compound paraquat, illustrating the importance of glutathione utilization in pneumococcal oxidative stress resistance. In addition, the gshT and gor mutant strains are hypersensitive to challenge with the divalent metal ions copper, cadmium, and zinc. The importance of glutathione utilization in pneumococcal colonization and invasion of the host is demonstrated by the attenuated phenotype of the gshT mutant strain in a mouse model of infection. |
Keywords: | Animals Mice Streptococcus pneumoniae Metals Glutathione Reductase Bacterial Proteins Membrane Transport Proteins Gene Expression Regulation, Bacterial Gene Expression Regulation, Enzymologic Oxidative Stress Mutation Time Factors |
Rights: | Copyright © 2012, American Society for Microbiology. All Rights Reserved |
DOI: | 10.1128/JB.01393-12 |
Published version: | http://dx.doi.org/10.1128/jb.01393-12 |
Appears in Collections: | Aurora harvest Molecular and Biomedical Science publications |
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