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https://hdl.handle.net/2440/76204
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Type: | Journal article |
Title: | Structure of the apo form of Bacillus Stearothermophilus Phosphofructokinase |
Author: | Mosser, R. Reddy, M. Bruning, J. Sacchettini, J. Reinhart, G. |
Citation: | Biochemistry, 2012; 51(3):769-775 |
Publisher: | Amer Chemical Soc |
Issue Date: | 2012 |
ISSN: | 0006-2960 1520-4995 |
Statement of Responsibility: | Rockann Mosser, Manchi C. M. Reddy, John B. Bruning, James C. Sacchettini, and Gregory D. Reinhart |
Abstract: | The crystal structure of the unliganded form of Bacillus stearothermophilus phosphofructokinase (BsPFK) was determined using molecular replacement to 2.8 Å resolution (Protein Data Bank entry 3U39 ). The apo BsPFK structure serves as the basis for the interpretation of any structural changes seen in the binary or ternary complexes. When the apo BsPFK structure is compared with the previously published liganded structures of BsPFK, the structural impact that the binding of the ligands produces is revealed. This comparison shows that the apo form of BsPFK resembles the substrate-bound form of BsPFK, a finding that differs from previous predictions. |
Keywords: | Phosphofructokinases Fructosephosphates Apoenzymes Bacterial Proteins Ligands Crystallography, X-Ray Allosteric Regulation Protein Binding Substrate Specificity Geobacillus stearothermophilus |
Rights: | © 2012 American Chemical Society |
DOI: | 10.1021/bi201548p |
Published version: | http://dx.doi.org/10.1021/bi201548p |
Appears in Collections: | Aurora harvest 4 Molecular and Biomedical Science publications |
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