Adelaide Research & Scholarship
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https://hdl.handle.net/2440/775
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| Type: | Journal article |
| Title: | Influence of broth dilution on the disruption of Escherichia coli |
| Author: | Kleinig, A. Mansell, C. Nguyen, Q. Badalyan, A. Middelberg, A. |
| Citation: | Biotechnology Letters, 1995; 9(10):759-762 |
| Publisher: | SPRINGER |
| Issue Date: | 1995 |
| ISSN: | 0951-208X 1573-6784 |
| Abstract: | The effect of cell concentration (5 to 150 g/L wet wt after broth dilution) on homogenizer disruption efficiency and homogenate viscosity is reported for E. coli. Broth dilution increases homogenizer efficiency and decreases feed and homogenate viscosity. However, this increase in disruption efficiency is not sufficient to warrant dilution of the broth prior to homogenization. The optimal feed concentration is the maximum possible that does not lead to practical handling difficulties due to high viscosity. © 1995 Chapman & Hall. |
| DOI: | 10.1007/BF00159244 |
| Published version: | http://dx.doi.org/10.1007/bf00159244 |
| Appears in Collections: | Aurora harvest 6 Chemical Engineering publications |
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