Adelaide Research & Scholarship
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https://hdl.handle.net/2440/776
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| Type: | Journal article |
| Title: | Quantitation of chloramphenicol acetyl transferase (CAT) messenger RNA by filter hybridisation using a digoxigenin label |
| Author: | Jorgensen, L. Middelberg, A. O'Neill, B. Thomas, C. |
| Citation: | Biotechnology Letters, 1996; 10(2):83-88 |
| Publisher: | CHAPMAN HALL LTD |
| Issue Date: | 1996 |
| ISSN: | 0951-208X 1573-6784 |
| Statement of Responsibility: | Lene Jorgensen, Anton P. J. Middelberg, Brian K. O'Neill, Connor J. Thomas |
| Abstract: | Slot- and dot-blotting are commonly used to evaluate levels of messenger ribonucleic acid (mRNA). Quantitation of bacterially-expressed chloramphenicol acetyl transferase (CAT) mRNA by this method is highly dependent on total RNA immobilised onto the solid support as well as mRNA concentration. mRNA quantitation by comparison with a pure standard results in underestimation. An improved protocol for CAT mRNA detection is described. |
| DOI: | 10.1007/BF00765187 |
| Published version: | http://dx.doi.org/10.1007/bf00765187 |
| Appears in Collections: | Aurora harvest 6 Chemical Engineering publications |
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