Please use this identifier to cite or link to this item:
https://hdl.handle.net/2440/78216
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Type: | Journal article |
Title: | Defective lung macrophage function in lung cancer±chronic obstructive pulmonary disease (COPD/emphysema)-mediated by cancer cell production of PGE2? |
Other Titles: | Defective lung macrophage function in lung cancer +/- chronic obstructive pulmonary disease (COPD/emphysema)-mediated by cancer cell production of PGE2? |
Author: | Dehle, F. Mukaro, V. Jurisevic, C. Moffat, D. Ahern, J. Hodge, G. Jersmann, H. Reynolds, P. Hodge, S. |
Citation: | PLoS One, 2013; 8(4):1-6 |
Publisher: | Public Library of Science |
Issue Date: | 2013 |
ISSN: | 1932-6203 1932-6203 |
Editor: | Tasken, K. |
Statement of Responsibility: | Francis C. Dehle, Violet R. Mukaro, Craig Jurisevic, David Moffat, Jessica Ahern, Greg Hodge, Hubertus Jersmann, Paul N. Reynolds, Sandra Hodge |
Abstract: | In chronic obstructive pulmonary disease (COPD/emphysema) we have shown a reduced ability of lung and alveolar (AM) macrophages to phagocytose apoptotic cells (defective ‘efferocytosis’), associated with evidence of secondary cellular necrosis and a resultant inflammatory response in the airway. It is unknown whether this defect is present in cancer (no COPD) and if so, whether this results from soluble mediators produced by cancer cells. We investigated efferocytosis in AM (26 controls, 15 healthy smokers, 37 COPD, 20 COPD+ non small cell lung cancer (NSCLC) and 8 patients with NSCLC without COPD) and tumor and tumor-free lung tissue macrophages (21 NSCLC with/13 without COPD). To investigate the effects of soluble mediators produced by lung cancer cells we then treated AM or U937 macrophages with cancer cell line supernatant and assessed their efferocytosis ability. We qualitatively identified Arachidonic Acid (AA) metabolites in cancer cells by LC-ESI-MSMS, and assessed the effects of COX inhibition (using indomethacin) on efferocytosis. Decreased efferocytosis was noted in all cancer/COPD groups in all compartments. Conditioned media from cancer cell cultures decreased the efferocytosis ability of both AM and U937 macrophages with the most pronounced effects occurring with supernatant from SCLC (an aggressive lung cancer type). AA metabolites identified in cancer cells included PGE2. The inhibitory effect of PGE2 on efferocytosis, and the involvement of the COX-2 pathway were shown. Efferocytosis is decreased in COPD/emphysema and lung cancer; the latter at least partially a result of inhibition by soluble mediators produced by cancer cells that include PGE2. |
Keywords: | Lung Cell Line, Tumor Subcellular Fractions Macrophages, Alveolar Bronchoalveolar Lavage Fluid Humans Lung Neoplasms Pulmonary Disease, Chronic Obstructive Pulmonary Emphysema Dinoprostone Demography Phagocytosis Adult Aged Middle Aged Female Male Cyclooxygenase 2 |
Rights: | © 2013 Dehle et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
DOI: | 10.1371/journal.pone.0061573 |
Grant ID: | NHMRC |
Published version: | http://dx.doi.org/10.1371/journal.pone.0061573 |
Appears in Collections: | Aurora harvest 4 Medicine publications |
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