Identification and expression analysis of TLPs as candidate genes promoting the responses to both biotic and abiotic stresses in wheat

Abstract

Thaumatin like protein (TLPs) gene family have the ability to respond to both biotic and abiotic stresses. In this research, gene expression and bioinformatics analysis were applied to reveal the function of TLPs more evidently. Real Time PCR technique was used to compare TLP gene expression between susceptible and resistant cultivars of wheat under the Mycosphaerella graminicola inoculation. Our results indicated the early resistant-related response by TLP up-regulation within 3h after inoculation. In abiotic stress we observed induction of TLP gene expression during salt stress in susceptible cultivar. 50mM NaCl induced rapid induction of TLP within 3h, while 100mM NaCl conferred TLP up-regulation later at 72h. In silico chromosome walking analysis revealed that four loci can be the candidates for conferring tolerance to both biotic and abiotic stresses. In this study, a combination of Real Time PCR technique and bioinformatics tools suggested the possible role of TLP homologs in response to both biotic and abiotic stresses.