Please use this identifier to cite or link to this item: https://hdl.handle.net/2440/82247
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Type: Journal article
Title: Transcriptional regulatory network analysis of the over-expressed genes in adipose tissue
Author: Bakhtiarizadeh, M.
Moradi-Shahrbabak, M.
Ebrahimie, E.
Citation: Genes and Genomics, 2014; 36(1):105-117
Publisher: Genetics Society of Korea
Issue Date: 2014
ISSN: 1976-9571
2092-9293
Statement of
Responsibility: 
Mohammad Reza Bakhtiarizadeh, Mohammad Moradi-Shahrbabak, Esmaeil Ebrahimie
Abstract: Adipose tissue plays important roles in whole body energy homeostasis and is now known to be a very important and active endocrine organ. The transcriptional regulatory network of adipose tissue metabolism is complex and much yet to be known. To identify transcriptional profile in adipose tissue, expressed sequence tag (EST) analysis using Digital Differential Display (DDD) was employed. The results of EST analysis were re-evaluated by microarray data using COXPRESdb (an available expression data repository). To uncover transcriptional regulatory mechanisms which play key roles in the adipose tissue metabolism, transcriptional regulatory network analysis was applied, using the promoter analysis and interaction network toolset. Sixty-five transcripts were found to be more frequent in adipose tissue in comparison to the other tissues. COXPRESdb result showed that 62 % of the identified over-expressed genes in adipose tissue by DDD had expression level greater than 1 (in base 2 logarithm). Based on coincidence of regulatory sites, candidate TFs were identified including TFs that previously known to be involved in adipose tissue metabolism (SP1, KROX, STAT1, LRF, VDR, LXR, SRF and HIF1) and TFs, such as CKROX, ZF5, ETF, AP-2, AP-2alpha, PAX-5, SPZ1, RBPJ and CACD, that had not been recognized previously. This work yielded several TF candidates activating in adipose tissue metabolism. These findings open a new avenue for future research on promoter occupancy and TF perturbation. © 2013 The Genetics Society of Korea.
Keywords: Promote
Adipose tissue
PAINT
Transcription factor
Rights: © The Genetics Society of Korea 2013
DOI: 10.1007/s13258-013-0145-x
Published version: http://dx.doi.org/10.1007/s13258-013-0145-x
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Molecular and Biomedical Science publications

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