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https://hdl.handle.net/2440/8521
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Type: | Journal article |
Title: | Efficient generation of [alpha](1,3) galactosyltransferase knockout porcine fetal fibroblasts for nuclear transfer |
Author: | Harrison, S. Guidolin, A. Faast, R. Crocker, L. Giannakis, C. d'Apice, A. Nottle, M. Lyons, I. |
Citation: | Transgenic Research, 2002; 11(2):143-150 |
Publisher: | Kluwer Academic Publ |
Issue Date: | 2002 |
ISSN: | 0962-8819 1573-9368 |
Statement of Responsibility: | Sharon J. Harrison, Angelo Guidolin, Renate Faast, Lesley A. Crocker, Chris Giannakis, Anthony J.F. d’Apice, Mark B. Nottle and Ian Lyons |
Abstract: | Pigs are currently considered themost likely source of organs for human xenotransplantation because of anatomical and physiological similarities to humans, and the relative ease with which they can be bred in large numbers. A severe form of rejection known as hyperacute rejection has been the major barrier to the use of xenografts. Generating transgenic pigs for organ transplantation is likely to involve precise genetic manipulation to ablate the α(1,3) galactosyltransferase (galT) gene. In contrast to the mouse, homologous recombination in livestock species to ablate genes is hampered by the inability to isolate functional embryonic stem cells. However, nuclear transfer using genetically targeted cultured somatic cells provides an alternativemeans to producing pigs deficient for galT. In this study we successfully produced galT+/− somatic porcine fetal fibroblasts using two approaches; positive negative selection (PNS) using an isogenic targeting construct, and with a promoterless vector using non-isogenic DNA. |
Keywords: | galT gene targeting isogenic transgenic |
Description: | The original publication is available at www.springerlink.com |
DOI: | 10.1023/A:1015262108526 |
Published version: | http://dx.doi.org/10.1023/a:1015262108526 |
Appears in Collections: | Aurora harvest Obstetrics and Gynaecology publications |
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