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https://hdl.handle.net/2440/85261
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Type: | Journal article |
Title: | Uterine epithelial cells synthesize granulocyte-macrophage colony-stimulating factor and interleukin-6 in pregnant and nonpregnant mice |
Author: | Robertson, S.A. Mayrhofer, G. Seamark, R.F. |
Citation: | Biology of Reproduction, 1992; 46(6):1069-1079 |
Publisher: | Society for the Study of Reproduction |
Issue Date: | 1992 |
ISSN: | 0006-3363 1529-7268 |
Statement of Responsibility: | Sarah A. Robertson, Graham Mayrhofer, and Robert F. Seamark |
Abstract: | Cytokine secretion by endometrial cells from estrous and mated mice was measured using specific bioassays. The granulocyte-macrophage colony-stimulating factor (GM-CSF) and interleukin-6 (IL-6) contents of uterine intraluminal fluid were elevated greater than 20-fold and 250-fold respectively following mating, and both cytokines were synthesized in abundance in vitro by uterine cells harvested at estrus and on Day 1 of pregnancy. Synthesis was not impaired in genetically lymphocyte-deficient nude, SCID, or beige mice. To determine the cellular origin of the cytokines, a panning technique employing monoclonal antibodies against a range of leukocyte and other lineage markers was used to isolate uterine cell subsets in vitro. These experiments identified glandular and/or luminal epithelial cells as the major source of GM-CSF and IL-6 in estrous and pregnant uteri. Stromal fibroblasts also synthesized IL-6, as did macrophages in mated mice. Epithelial cells harvested from midgestation uteri secreted GM-CSF and IL-6 in quantities similar to those of cells from estrous and mated mice. Bioactivities of both cytokines derived from epithelial cells were neutralized by specific antibodies, and size-exclusion chromatography of conditioned media from uterine cells revealed peaks of GM-CSF and IL-6 bioactivity with M(r) 23,000 and 23,000-26,000, respectively. Bioassay of luminal fluids and culture supernatants were negative for the cytokines interleukin-1, interleukin-2, interleukin-3, and tumor necrosis factor-alpha. These studies identify murine uterine epithelium as a potent source of the cytokines GM-CSF and IL-6, which we postulate have potentially important functions in pregnancy through actions on target cells in both the uterus and the conceptus. |
Keywords: | Uterus Endometrium Epithelium Cells, Cultured Animals Mice, Inbred BALB C Mice, Inbred C57BL Mice, Inbred CBA Mice Mice, Nude Mice, SCID Granulocyte-Macrophage Colony-Stimulating Factor Interleukin-6 Culture Media Immunohistochemistry Estrus Pregnancy Pregnancy, Animal Molecular Weight Female |
Rights: | Copyright status unknown |
DOI: | 10.1095/biolreprod46.6.1069 |
Published version: | http://dx.doi.org/10.1095/biolreprod46.6.1069 |
Appears in Collections: | Aurora harvest 7 Obstetrics and Gynaecology publications |
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