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https://hdl.handle.net/2440/86668
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dc.contributor.author | Jilbert, A.R. | - |
dc.contributor.author | Hertzog, P.J. | - |
dc.contributor.author | Burrell, C.J. | - |
dc.contributor.author | Gowans, E.J. | - |
dc.contributor.author | Linnane, A.W. | - |
dc.contributor.author | Marmion, B.P. | - |
dc.date.issued | 1986 | - |
dc.identifier.citation | Microbial Pathogenesis, 1986; 1(2):159-168 | - |
dc.identifier.issn | 0882-4010 | - |
dc.identifier.issn | 1096-1208 | - |
dc.identifier.uri | http://hdl.handle.net/2440/86668 | - |
dc.description.abstract | A monoclonal antibody to human interferon-alpha (IFN-alpha) was produced using affinity-purified IFN-alpha, that reacted with recombinant human IFN-alpha 2, but not with IFN-alpha 1, IFN-alpha M1 or IFN-beta. Indirect immunofluorescence using this monoclonal (designated 6C3) and anti-IFN-alpha polyclonal antibodies identified cells expressing IFN-alpha. After Sendai virus induction of normal human buffy-coat cells the proportion of monocytes and lymphocytes expressing IFN-alpha rose progressively from 0% to 50% and 34% respectively, preceding peak IFN-alpha titres in the culture supernatants. Around 80-90% of polymorphs were IFN-alpha-positive using both antisera, with or without IFN induction, although very little IFN bioactivity was released to the supernatant of polymorph cultures after IFN induction. Sections of hepatitis B virus infected human liver tissue showed foci of IFN-alpha-positive infiltrating mononuclear cells and (to a lesser extent) fibroblasts in patients who had active cirrhosis and evidence of virus replication. These findings suggest that polymorphs constitutively express IFN-alpha 2 related antigenic activity, whose biological activity is at present unknown; and demonstrates the identification of IFN-alpha-expressing cells in sections of tissue undergoing natural virus infection. | - |
dc.description.statementofresponsibility | Allison R. Jilbert, Paul J. Hertzog, Christopher J. Burrell, Eric J. Gowans, Anthony W. Linnane, Barrie P. Marmion | - |
dc.language.iso | en | - |
dc.publisher | Elsevier | - |
dc.rights | ©1986 Academic Press Inc. (London) Ltd. | - |
dc.source.uri | http://dx.doi.org/10.1016/0882-4010(86)90018-5 | - |
dc.subject | human interferon-α; monoclonal antibodies; immunofluorescence; hepatitis B virus infection | - |
dc.title | Production of a monoclonal antibody to human interferon-α (IFN-α) and its use to identify IFN-α-producing cells in virus infection in vivo | - |
dc.title.alternative | Production of a monoclonal antibody to human interferon-alpha (IFN-alpha) and its use to identify IFN-alpha-producing cells in virus infection in vivo | - |
dc.type | Journal article | - |
dc.identifier.doi | 10.1016/0882-4010(86)90018-5 | - |
pubs.publication-status | Published | - |
dc.identifier.orcid | Jilbert, A.R. [0000-0003-3855-1679] | - |
dc.identifier.orcid | Burrell, C.J. [0000-0002-4020-349X] | - |
dc.identifier.orcid | Gowans, E.J. [0000-0002-4274-8311] | - |
Appears in Collections: | Aurora harvest 7 Molecular and Biomedical Science publications |
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