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https://hdl.handle.net/2440/8917
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Type: | Journal article |
Title: | Heteronuclear ribonucleoproteins C1 ad C2, components of the spliceosome, are specific targets of interleukin 1β-converting enzyme-like proteases in apoptosis |
Other Titles: | Heteronuclear ribonucleoproteins C1 ad C2, components of the spliceosome, are specific targets of interleukin 1beta-converting enzyme-like proteases in apoptosis |
Author: | Waterhouse, N. Kumar, S. Song, Q. Strike, P. Sparrow, L. Dreyfuss, G. Alnemri, E. Litwack, G. Lavin, M. Watters, D. |
Citation: | Journal of Biological Chemistry, 1996; 271(46):29335-29341 |
Publisher: | AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC |
Issue Date: | 1996 |
ISSN: | 0021-9258 1083-351X |
Abstract: | Apoptosis induced by a variety of agents results in the proteolytic cleavage of a number of cellular substrates by enzymes related to interleukin 1β-converting enzyme (ICE). A small number of substrates for these enzymes have been identified to date, including enzymes involved in DNA repair processes: poly(ADP-ribose) polymerase and DNA-dependent protein kinase. We describe here for the first time the specific cleavage of the heteronuclear ribonucleoproteins (hnRNPs) C1 and C2 in apoptotic cells induced to undergo apoptosis by a variety of stimuli, including ionizing radiation, etoposide, and ceramide. No cleavage was observed in cells that are resistant to apoptosis induced by ionizing radiation. Protease inhibitor data implicate the involvement of an ICE-like protease in the cleavage of hnRNP C. Using recombinant ICE-like proteases and purified hnRNP C proteins in vitro, we show that the C proteins are cleaved by Mch3α and CPP32 and, to a lesser extent, by Mch2α, but not by ICE, Nedd2, Tx, or the cytotoxic T-cell protease granzyme B. The results described here demonstrate that the hnRNP C proteins, abundant nuclear proteins thought to be involved in RNA splicing, belong to a critical set of protein substrates that are cleaved by ICE-like proteases during apoptosis. |
Keywords: | Tumor Cells, Cultured Spliceosomes Humans Cysteine Endopeptidases Caspase 1 Ribonucleoproteins Heterogeneous-Nuclear Ribonucleoproteins Heterogeneous-Nuclear Ribonucleoprotein Group C Apoptosis Substrate Specificity Hydrolysis Radiation Tolerance |
Description: | ©1996 by The American Society for Biochemistry and Molecular Biology, Inc. |
DOI: | 10.1074/jbc.271.46.29335 |
Published version: | http://www.jbc.org/cgi/content/abstract/271/46/29335 |
Appears in Collections: | Aurora harvest Medicine publications |
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