Please use this identifier to cite or link to this item:
https://hdl.handle.net/2440/93740
Citations | ||
Scopus | Web of Science® | Altmetric |
---|---|---|
?
|
?
|
Type: | Journal article |
Title: | Low-pH and aluminum resistance in Arabidopsis correlates with high cytosolic magnesium content and increased magnesium uptake by plant roots |
Author: | Bose, J. Babourina, O. Shabala, S. Rengel, Z. |
Citation: | Plant and Cell Physiology, 2013; 54(7):1093-1104 |
Publisher: | Oxford University Press |
Issue Date: | 2013 |
ISSN: | 0032-0781 1471-9053 |
Statement of Responsibility: | Jayakumar Bose, Olga Babourina, Sergey Shabala, and Zed Rengel |
Abstract: | Low-pH stress and Al3+ toxicity affect root growth in acid soils. It was hypothesized that the capacity of genotypes to maintain Mg2+ uptake in acidic environments may contribute to low-pH and Al resistance, but explicit evidence is lacking. In this work, an Al-resistant alr104 mutant and two Al-sensitive mutants (als5 and als3) of Arabidopsis thaliana were compared with the wild type (Col-0) for Mg2+ uptake and intracellular Mg2+ concentration under low-pH and combined low-pH/Al stresses. Magnesium accumulation in roots was measured in long-term (7 d) experiments. The Mg2+ fluxes were measured using ion-sensitive microelectrodes at the distal elongation and the mature root zones in short-term (0–60 min) experiments. Intracellular Mg2+ concentrations were measured in intact root cells at the distal elongation zone using magnesium-specific fluorescent dye and fluorescent lifetime imaging (FLIM) analysis. Under low-pH stress, Arabidopsis mutants als5 and alr104 maintained a higher Mg concentration in roots, and had greater Mg2+ influx than the wild type and the als3 mutant. Under combined low-pH/Al treatment, Al-resistant genotypes (wild type and alr104) maintained a higher Mg2+ accumulation, and had a higher Mg2+ influx and higher intracellular Mg2+ concentration than Al-sensitive genotypes (als3 and als5). Overall, these results show that increased Mg2+ uptake correlates with an enhanced capacity of Arabidopsis genotypes to cope with low-pH and combined low-pH/Al stresses. |
Keywords: | Aluminum toxicity Arabidopsis thaliana Intracellular magnesium Low pH Magnesium flux |
Rights: | ©The Author 2013 |
DOI: | 10.1093/pcp/pct064 |
Grant ID: | ARC |
Published version: | http://dx.doi.org/10.1093/pcp/pct064 |
Appears in Collections: | Agriculture, Food and Wine publications Aurora harvest 2 |
Files in This Item:
There are no files associated with this item.
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.