Please use this identifier to cite or link to this item:
https://hdl.handle.net/2440/103845
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Type: | Journal article |
Title: | Biotin protein ligase is a target for new antibacterials |
Author: | Feng, J. Paparella, A.S. Booker, G.W. Polyak, S.W. Abell, A.D. |
Citation: | Antibiotics, 2016; 5(3):26-1-26-15 |
Publisher: | MDPI |
Issue Date: | 2016 |
ISSN: | 2079-6382 2079-6382 |
Statement of Responsibility: | Jiage Feng, Ashleigh S. Paparella, Grant W. Booker, Steven W. Polyak, and Andrew D. Abell |
Abstract: | There is a desperate need for novel antibiotic classes to combat the rise of drug resistant pathogenic bacteria, such as Staphylococcus aureus. Inhibitors of the essential metabolic enzyme biotin protein ligase (BPL) represent a promising drug target for new antibacterials. Structural and biochemical studies on the BPL from S. aureus have paved the way for the design and development of new antibacterial chemotherapeutics. BPL employs an ordered ligand binding mechanism for the synthesis of the reaction intermediate biotinyl-5'-AMP from substrates biotin and ATP. Here we review the structure and catalytic mechanism of the target enzyme, along with an overview of chemical analogues of biotin and biotinyl-5'-AMP as BPL inhibitors reported to date. Of particular promise are studies to replace the labile phosphoroanhydride linker present in biotinyl-5'-AMP with alternative bioisosteres. A novel in situ click approach using a mutant of S. aureus BPL as a template for the synthesis of triazole-based inhibitors is also presented. These approaches can be widely applied to BPLs from other bacteria, as well as other closely related metabolic enzymes and antibacterial drug targets. |
Keywords: | Staphylococcus aureus X-ray crystallography antibiotic biotin biotin protein ligase in situ click chemistry inhibitor design |
Rights: | © 2016 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC-BY) license (http://creativecommons.org/licenses/by/4.0/). |
DOI: | 10.3390/antibiotics5030026 |
Grant ID: | http://purl.org/au-research/grants/nhmrc/1068885 |
Published version: | http://dx.doi.org/10.3390/antibiotics5030026 |
Appears in Collections: | Aurora harvest 7 Molecular and Biomedical Science publications |
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hdl_103845.pdf | Published version | 1.72 MB | Adobe PDF | View/Open |
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