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https://hdl.handle.net/2440/11635
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DC Field | Value | Language |
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dc.contributor.author | Proudman, S. | - |
dc.contributor.author | Cleland, L. | - |
dc.contributor.author | Mayrhofer, G. | - |
dc.date.issued | 1999 | - |
dc.identifier.citation | Journal of Rheumatology, 1999; 26(9):1877-1889 | - |
dc.identifier.issn | 0315-162X | - |
dc.identifier.issn | 1499-2752 | - |
dc.identifier.uri | http://hdl.handle.net/2440/11635 | - |
dc.description.abstract | <h4>Objective</h4>To examine expression of adhesion molecules within human synovial xenografts in vivo after injection of human cytokines or peripheral blood mononuclear cells (PBMC).<h4>Methods</h4>Rheumatoid synovium was transplanted into subcutaneous pouches in the ears of severe combined immunodeficient (SCID) mice. Tumor necrosis factor-alpha (TNF-alpha), interleukin 1beta (IL-1beta), or PBMC were injected into the xenografts. The grafts were assessed by immunohistochemistry and quantitative video image analysis.<h4>Results</h4>TNF-alpha, IL-1beta, and concanavalin A activated PBMC increased the expression of vascular cellular adhesion molecule 1 and intercellular adhesion molecule 1 significantly on vessels and nonvascular synovial cells compared with injection of medium only. Vascular expression of E-selectin, but not of MHC Class II, was also increased. These stimuli also induced the migration of murine leukocytes into the xenografts.<h4>Conclusion</h4>Rheumatoid synovial xenografts in SCID mice respond by upregulation of adhesion molecule expression when challenged in vivo with proinflammatory cytokines or activated PBMC. | - |
dc.language.iso | en | - |
dc.publisher | J RHEUMATOL PUBL CO | - |
dc.subject | Synovial Membrane | - |
dc.subject | Leukocytes, Mononuclear | - |
dc.subject | Animals | - |
dc.subject | Humans | - |
dc.subject | Mice | - |
dc.subject | Mice, SCID | - |
dc.subject | Arthritis, Rheumatoid | - |
dc.subject | Disease Models, Animal | - |
dc.subject | Tumor Necrosis Factor-alpha | - |
dc.subject | Intercellular Adhesion Molecule-1 | - |
dc.subject | Vascular Cell Adhesion Molecule-1 | - |
dc.subject | Interleukin-1 | - |
dc.subject | Transplantation, Heterologous | - |
dc.subject | Immunohistochemistry | - |
dc.subject | Injections, Intralesional | - |
dc.subject | Sensitivity and Specificity | - |
dc.subject | Cell Movement | - |
dc.subject | Up-Regulation | - |
dc.title | Effects of TNF-a, IL-1b and activated peripheral blood mononuclear cells on the expression of adhesion molecules and recruitment of leukocytes in rheumatoid synovial xenografts in SCID mice. | - |
dc.type | Journal article | - |
pubs.publication-status | Published | - |
dc.identifier.orcid | Proudman, S. [0000-0002-3046-9884] | - |
Appears in Collections: | Aurora harvest 7 Microbiology and Immunology publications |
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