Please use this identifier to cite or link to this item:
https://hdl.handle.net/2440/11635
Type: | Journal article |
Title: | Effects of TNF-a, IL-1b and activated peripheral blood mononuclear cells on the expression of adhesion molecules and recruitment of leukocytes in rheumatoid synovial xenografts in SCID mice. |
Author: | Proudman, S. Cleland, L. Mayrhofer, G. |
Citation: | Journal of Rheumatology, 1999; 26(9):1877-1889 |
Publisher: | J RHEUMATOL PUBL CO |
Issue Date: | 1999 |
ISSN: | 0315-162X 1499-2752 |
Abstract: | <h4>Objective</h4>To examine expression of adhesion molecules within human synovial xenografts in vivo after injection of human cytokines or peripheral blood mononuclear cells (PBMC).<h4>Methods</h4>Rheumatoid synovium was transplanted into subcutaneous pouches in the ears of severe combined immunodeficient (SCID) mice. Tumor necrosis factor-alpha (TNF-alpha), interleukin 1beta (IL-1beta), or PBMC were injected into the xenografts. The grafts were assessed by immunohistochemistry and quantitative video image analysis.<h4>Results</h4>TNF-alpha, IL-1beta, and concanavalin A activated PBMC increased the expression of vascular cellular adhesion molecule 1 and intercellular adhesion molecule 1 significantly on vessels and nonvascular synovial cells compared with injection of medium only. Vascular expression of E-selectin, but not of MHC Class II, was also increased. These stimuli also induced the migration of murine leukocytes into the xenografts.<h4>Conclusion</h4>Rheumatoid synovial xenografts in SCID mice respond by upregulation of adhesion molecule expression when challenged in vivo with proinflammatory cytokines or activated PBMC. |
Keywords: | Synovial Membrane Leukocytes, Mononuclear Animals Humans Mice Mice, SCID Arthritis, Rheumatoid Disease Models, Animal Tumor Necrosis Factor-alpha Intercellular Adhesion Molecule-1 Vascular Cell Adhesion Molecule-1 Interleukin-1 Transplantation, Heterologous Immunohistochemistry Injections, Intralesional Sensitivity and Specificity Cell Movement Up-Regulation |
Appears in Collections: | Aurora harvest 7 Microbiology and Immunology publications |
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