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https://hdl.handle.net/2440/45285
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Type: | Journal article |
Title: | CreA-mediated repression in Aspergillus nidulans does not require transcriptional auto-regulation, regulated intracellular localisation or degradation of CreA |
Author: | Roy, P. Lockington, R. Kelly, J. |
Citation: | Fungal Genetics and Biology, 2008; 45(5):657-670 |
Publisher: | Academic Press Inc Elsevier Science |
Issue Date: | 2008 |
ISSN: | 1087-1845 1096-0937 |
Statement of Responsibility: | Preeti Roy, Robin A. Lockington and Joan M. Kelly |
Abstract: | The major regulatory protein in carbon repression in Aspergillus nidulans is CreA. Strains constitutively over-expressing creA show normal responses to carbon repression, indicating that auto-regulation of creA is not essential for CreA-mediated regulation. In these strains, high levels of CreA are present whether cells are grown in repressing or derepressing conditions, indicating large-scale degradation of CreA does not play a key role. CreA is located in the nucleus and cytoplasm in cells when grown in either repressing or derepressing conditions, and absence of CreB, CreD or AcrB does not affect either the localisation or amount of CreA. Therefore, CreA must require some modification or interaction to act as a repressor. Deletion analysis indicates that a region of CreA thought to be important for repression in Trichoderma reesei and Sclerotina sclerotiorum CreA homologues is not critical for function in Aspergillus nidulans. |
Keywords: | Cell Nucleus Cytoplasm Ascomycota Aspergillus nidulans Trichoderma Carbon Fungal Proteins Green Fluorescent Proteins Recombinant Fusion Proteins Repressor Proteins Microscopy, Fluorescence Blotting, Western DNA Mutational Analysis Gene Expression Regulation, Fungal Gene Deletion Mutation Genes, Reporter |
Description: | Copyright © 2007 Elsevier Inc. All rights reserved. |
Provenance: | Available online 1 November 2007. |
DOI: | 10.1016/j.fgb.2007.10.016 |
Description (link): | http://www.elsevier.com/wps/find/journaldescription.cws_home/622835/description#description |
Published version: | http://dx.doi.org/10.1016/j.fgb.2007.10.016 |
Appears in Collections: | Aurora harvest Molecular and Biomedical Science publications |
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