Please use this identifier to cite or link to this item:
https://hdl.handle.net/2440/76951
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Type: | Journal article |
Title: | A physical, genetic and functional sequence assembly of the barley genome |
Author: | Langridge, P. Shi, B. Fincher, G. |
Citation: | Nature, 2012; 491(7426):711-717 |
Publisher: | Nature Publishing Group |
Issue Date: | 2012 |
ISSN: | 0028-0836 1476-4687 |
Contributor: | Langridge, Peter Shi, Bu-jun Fincher, Geoffrey |
Statement of Responsibility: | The International Barley Genome Sequencing Consortium |
Abstract: | Barley (Hordeum vulgare L.) is among the world’s earliest domesticated and most important crop plants. It is diploid with a large haploid genome of 5.1 gigabases (Gb). Here we present an integrated and ordered physical, genetic and functional sequence resource that describes the barley gene-space in a structured whole-genome context. We developed a physical map of 4.98 Gb, with more than 3.90 Gb anchored to a high-resolution genetic map. Projecting a deep whole-genome shotgun assembly, complementary DNA and deep RNA sequence data onto this framework supports 79,379 transcript clusters, including 26,159 ‘high-confidence’ genes with homology support from other plant genomes. Abundant alternative splicing, premature termination codons and novel transcriptionally active regions suggest that post-transcriptional processing forms an important regulatory layer. Survey sequences from diverse accessions reveal a landscape of extensive single-nucleotide variation. Our data provide a platform for both genome-assisted research and enabling contemporary crop improvement. |
Keywords: | International Barley Genome Sequencing Consortium Hordeum Crops, Agricultural Codon, Nonsense Physical Chromosome Mapping Sequence Analysis, DNA Genomics Evolution, Molecular Gene Expression Regulation, Plant Alternative Splicing Repetitive Sequences, Nucleic Acid Polymorphism, Single Nucleotide Genes, Plant Genome, Plant Molecular Sequence Annotation Transcriptome |
Description: | University of Adelaide contributors: Peter Langridge & Bu-jun Shi for Australian Centre for Plant Functional Genomics, Geoffrey Fincher for ARC Centre of Excellence in Plant Cell Walls |
Rights: | This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 3.0 Unported License. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-sa/3.0/ |
DOI: | 10.1038/nature11543 |
Published version: | http://dx.doi.org/10.1038/nature11543 |
Appears in Collections: | Agriculture, Food and Wine publications Aurora harvest 4 |
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hdl_76951.pdf | Published version | 1.16 MB | Adobe PDF | View/Open |
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